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rabbit anti fndc5  (Proteintech)


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    Proteintech rabbit anti fndc5
    Rabbit Anti Fndc5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+fndc5/pmc12968114-88-27-35?v=Proteintech
    Average 93 stars, based on 47 article reviews
    rabbit anti fndc5 - by Bioz Stars, 2026-07
    93/100 stars

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    ( A ) The experimental procedure employed in this study. The intestinal expression of <t>irisin/FNDC5</t> in each group. ( B ) Western blot band of recombinant irisin and Anti-FNDC5 antibody binding, the samples are triplicates. ( C and D ) Western blot and its analysis of the expression irisin/FNDC5. ( E ) Representative photos of frozen section immunofluorescence staining of the expression irisin/FNDC5 (green) and corresponding nuclear counterstaining (blue) in the intestine. Scale bar = 100 μm. *P < 0.05, vs Ctrl group; ## P < 0.01, vs LPS group.
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    ( A ) The experimental procedure employed in this study. The intestinal expression of irisin/FNDC5 in each group. ( B ) Western blot band of recombinant irisin and Anti-FNDC5 antibody binding, the samples are triplicates. ( C and D ) Western blot and its analysis of the expression irisin/FNDC5. ( E ) Representative photos of frozen section immunofluorescence staining of the expression irisin/FNDC5 (green) and corresponding nuclear counterstaining (blue) in the intestine. Scale bar = 100 μm. *P < 0.05, vs Ctrl group; ## P < 0.01, vs LPS group.

    Journal: Journal of Inflammation Research

    Article Title: Protective Effects of Irisin Against LPS-Induced Intestinal Injury and Its Influence on Gut Microbiota in Septic Mice

    doi: 10.2147/JIR.S542608

    Figure Lengend Snippet: ( A ) The experimental procedure employed in this study. The intestinal expression of irisin/FNDC5 in each group. ( B ) Western blot band of recombinant irisin and Anti-FNDC5 antibody binding, the samples are triplicates. ( C and D ) Western blot and its analysis of the expression irisin/FNDC5. ( E ) Representative photos of frozen section immunofluorescence staining of the expression irisin/FNDC5 (green) and corresponding nuclear counterstaining (blue) in the intestine. Scale bar = 100 μm. *P < 0.05, vs Ctrl group; ## P < 0.01, vs LPS group.

    Article Snippet: Rabbit anti-mouse FNDC5, occludin, caspase-3, and Bax antibodies were obtained from Wuhan Sanying Biotechnology Co., Ltd. Rabbit anti-mouse Bcl-2 antibody was purchased from Abcam, UK.

    Techniques: Expressing, Western Blot, Recombinant, Binding Assay, Immunofluorescence, Staining

    ( A ) The intestinal apoptosis of mice in each group was detected by TUNEL fluorescent staining. Nuclei were labeled with blue fluorescence (DAPI), and cell apoptosis was labeled with green fluorescence. Scale bar = 100 μm. ( B ) The density of TUNEL-positive cells was analyzed. ( C ) Irisin alleviated bacteremia in septic mice, as assessed by incubating peripheral blood on a blood agar plate. The black arrows in the figure indicate the bacterial colonies. ( D ) The CFU of bacteremia were count and analyzed (CFU/mL). *P < 0.05, **P < 0.01, vs Ctrl group; # P < 0.05, ## P < 0.01, vs LPS group. ( E ) The correlation between endogenous irisin/FNDC5 and various biomarkers in serum and intestinal tissue was evaluated. The Spearman correlation coefficient, which ranges from −1 to 1, is depicted in varying shades of blue, transitioning from light to dark. Specific values are marked in the respective areas, indicating the direction and strength of the correlation between the two variables. The triangles, both black and white, signify that the P-values for these correlations are statistically significant ( ▲ P < 0.05, ▲▲ P < 0.01).

    Journal: Journal of Inflammation Research

    Article Title: Protective Effects of Irisin Against LPS-Induced Intestinal Injury and Its Influence on Gut Microbiota in Septic Mice

    doi: 10.2147/JIR.S542608

    Figure Lengend Snippet: ( A ) The intestinal apoptosis of mice in each group was detected by TUNEL fluorescent staining. Nuclei were labeled with blue fluorescence (DAPI), and cell apoptosis was labeled with green fluorescence. Scale bar = 100 μm. ( B ) The density of TUNEL-positive cells was analyzed. ( C ) Irisin alleviated bacteremia in septic mice, as assessed by incubating peripheral blood on a blood agar plate. The black arrows in the figure indicate the bacterial colonies. ( D ) The CFU of bacteremia were count and analyzed (CFU/mL). *P < 0.05, **P < 0.01, vs Ctrl group; # P < 0.05, ## P < 0.01, vs LPS group. ( E ) The correlation between endogenous irisin/FNDC5 and various biomarkers in serum and intestinal tissue was evaluated. The Spearman correlation coefficient, which ranges from −1 to 1, is depicted in varying shades of blue, transitioning from light to dark. Specific values are marked in the respective areas, indicating the direction and strength of the correlation between the two variables. The triangles, both black and white, signify that the P-values for these correlations are statistically significant ( ▲ P < 0.05, ▲▲ P < 0.01).

    Article Snippet: Rabbit anti-mouse FNDC5, occludin, caspase-3, and Bax antibodies were obtained from Wuhan Sanying Biotechnology Co., Ltd. Rabbit anti-mouse Bcl-2 antibody was purchased from Abcam, UK.

    Techniques: TUNEL Assay, Staining, Labeling, Fluorescence